Date of Graduation

5-2015

Document Type

Thesis

Degree Name

Bachelor of Science in Biological Engineering

Degree Level

Undergraduate

Department

Biological and Agricultural Engineering

Advisor

Carrier, Danielle Julie

Abstract

Short leaf and loblolly pine were harvested in May 2014 and June 2014, respectively, at the University of Arkansas Monticello in Monticello, Arkansas. Short leaf and loblolly essential oils were prepared by hydrodistillation, using a Clevenger apparatus. Essential oils were extracted at separate times and showed different concentrations of the components α-pinene, β- pinene, 3-carene, limonene, and terpineol. Pine essential oil samples were tested through disc diffusion assay for antimicrobial activity against Listeria monocytogenes. This was done by placing ten μL of each essential oil sample on a paper disc on an agar plate that was inoculated with L. monocytogenes at a concentration of 3.7 x 10⁸ cells. Four of the five oils tested showed antimicrobial activity against L. monocytogenes and a liquid byproduct of hydrodistillation did not show any activity.

Keywords

hydrodistillation, essential oil, antimicrobial, listeria monocytogenes

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