Date of Graduation

5-2015

Document Type

Thesis

Degree Name

Bachelor of Science in Biomedical Engineering

Degree Level

Undergraduate

Department

Biomedical Engineering

Advisor

Wolchok, Jeffrey C

Abstract

In order to lengthen the functional time of implantable ECM, the mechanism of degradation must be inhibited. The matrix metalloproteases are the primary method by which ECM is broken down, and the TIMP family of proteins exists as proof that inhibition of these proteases is possible naturally. In particular, the glycoprotein TIMP-1 plays an inhibitory role with most of the MMPs including the collagenases. Furthermore, there exist synthetic or chemical molecules that likewise inhibit the catalytic actions of MMPs. One such molecule is Batimastat (BB-94) which has been proven to be a dose dependent inhibitor of MMPs during the inflammatory response. In order to evaluate the potential to increase the effective time of ECM based implants, an in vitro assay was developed to monitor the natural degradation rate of ECM in culture with DQ collagen and 3T3 fibroblasts. The protein TIMP-1 and the molecule BB-94 were incorporated into this model to evaluate their potential use in controlling the degradation rate of ECM implants.

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