Date of Graduation

5-2016

Document Type

Thesis

Degree Name

Bachelor of Science in Chemical Engineering

Degree Level

Undergraduate

Department

Chemical Engineering

Advisor

Beitle, Robert

Reader

Hestekin, Christa

Abstract

Current methods of detecting waterborne pathogens involve testing strips which take 24-48 hours to yield results, or require expensive equipment in order to function. In minimalist environments, these two technologies are not always applicable to test water quality. With the emergence of a new method of PCR, named LAMP PCR, it is possible to quickly and accurately detect pathogen DNA in a water sample. In order to scale this technique into a simple device, the aspects of the reaction must be accommodated, and a visual detection method chosen. A handheld device which keeps the isothermal LAMP PCR stable for the desired time was created to meet the former objective, and a fluorescent dye known as EvaGreen was employed for the latter.