Protein isolates and peptide fractions from food sources (cereal grains), have been shown to exert bioactive properties including antiobesity, anticancer, antiangiogenic, etc. One such food source is rice bran, which is an underutilized co-product of rough rice milling. It contains 90% of the nutrients and nutraceuticals of value to health, including high quality protein. The high quality protein is a potential source to generate peptides that can reduce hypertension and oxidative stress, both being important risk factors for cardiovascular diseases. The objective of this study was to extract peptide hydrolysates from heat stabilized defatted rice bran by enzymatic hydrolysis, evaluate the hydrolysates for gastrointestinal (GI) resistance, fractionate the GI-resistant hydrolysates by ultrafiltration to obtain >50 and 10-50 kDa fractions, and determine antihypertensive and antioxidant activities in the fractions. For antihypertension activity, angiotension-1 converting enzyme (ACE) assay, and for antioxidant activity, the 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) assay was conducted. We report that the ACE-I inhibition activity values for the unfractionated and unhydrolyzed (control), and fractions of >50 kDa, and 10-50 kDa were 6% (control), 78%, and 55%, respectively, clearly denoting antihypertensive activity for the peptide fractions. When tested for antioxidant activity, the >50 kDa fraction decreased from an initial DPPH of 95.48 to 78.99 mg/g, while the 10-50 kDa fraction decreased from an initial 110.35 to 76.53 mg/g, depicting reduction of radical-induced oxidant stress. The results demonstrated that the high molecular sized peptide hydrolysate fractions (>50 and 10-50 kDa) from rice bran bear antihypertensive and antioxidant properties and could possibly find a place as a health beneficial nutraceutical ingredient in food applications.
Hull, Janika; Kannan, Arvind; and Hettiarachchy, Navam S.
"Antioxidant and antihypertensive activities of rice bran peptides,"
Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences. University of Arkansas System Division of Agriculture. 12:52-57.
Available at: http://scholarworks.uark.edu/discoverymag/vol12/iss1/10