Cancer is a common cause of death in the United States and locally in the state of Arkansas. Modifiable factors such as tobacco use, physical activity, and diet lead to reduced incidence of colon cancer diagnosis. Plant-based foods may contain phytochemicals that confer health promoting properties. Specifically, peanuts contain phytochemicals known as resveratrol, arachidin-1, and arachidin-3 that have been linked to anticancer activities. However, few research studies have been done on arachidin-1 and arachidin-3 that could develop understanding of their health promoting properties or nutraceutical applications. The objectives of this study were to (1) determine the most effective concentration of arachidin-1 and arachidin-3 for inhibiting cell proliferation and (2) assess the bioavailability of these compounds. Concentrations of 0, 5, 10, and 20 µM arachidin-1 and arachidin-3 were applied and cell viability was measured at 0, 24, and 48 hours. Significant reduction of cell proliferation occurred with treatments of 10 and 20 µM arachidin-1 and 10 and 20 µM arachidin-3 in comparison with the control. Due to the limitations of high performance liquid chromatography (HPLC) detection, no transport values were determined when arachidin-1 and arachidin-3 were applied in 50 and 100 µM concentrations. The findings suggest that arachidin-1 and arachidin-3 inhibit cell proliferation in human colon cancer cells. Further research is needed to understand the bioavailability of arachidin-1 and arachidin-3.
Shirley, Macy R.; Brownmiller, Cindi; Lam, Wing Shun; and Lee, Sun-Ok
"Anti-Cancer and Bioavailabilty of Arachidin-1 and Arachidin-3 in Colon Cancer Cells,"
Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences. University of Arkansas System Division of Agriculture. 18:73-80.
Available at: http://scholarworks.uark.edu/discoverymag/vol18/iss1/13