Date of Graduation

8-2016

Document Type

Dissertation

Degree Name

Doctor of Philosophy in Food Science (PhD)

Degree Level

Graduate

Department

Food Science

Advisor

Philip G. Crandall

Committee Member

Steven Ricke

Second Committee Member

Michael Johnson

Third Committee Member

Young-Min Kwon

Fourth Committee Member

Jim Rankin

Keywords

Biological sciences, Listeria, Starvation, Viable but non-culturable

Abstract

Listeria spp. are often isolated from raw and processed food products. Listeria monocytogenes is responsible for death rates of 10 to 30% in immune compromised people; it can also infect a vast range of animals. There are numerous reports of poultry harboring or becoming infected with L. monocytogenes. First, this research assessed the ability of L. monocytogenes to replicate within chicken macrophages. Using the gentamicin-killing assay, a cell culture of chicken macrophage-like cells (HD11) were infected with two strains of L. monocytogenes individually (EGD-e and Scott A). Results show that unlike data on mouse macrophages, intracellular L. monocytogenes do not reduce (P

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