Date of Graduation
Doctor of Philosophy in Engineering (PhD)
Second Committee Member
Third Committee Member
Fourth Committee Member
Pure sciences; Applied sciences; ATRP; Biologics; Downstream process; Membrane chromatography; Protein separations; Surface modification
Membrane chromatography, or membrane adsorber, represents an attractive alternative to conventional packed bed chromatography used in downstream processing. Membrane chromatography has many advantages, including high productivity, low buffer consumption and ease to scale up. This doctoral dissertation focuses on developing novel polymeric ligands for protein separations using membrane chromatography. Atom transfer radical polymerization (ATRP), known as a controlled radical polymerization technique, has been used to control the architecture of grafted polymeric ligands. The center theme of this dissertation is to develop new polymeric ligands and investigate how the polymer’s property (e.g. flexibility, hydrophobicity) and architecture (e.g. chain density, chain length) affect the protein separation performances.
Liu, Zizhao, "Membrane Chromatography for Bioseparations: Ligand Design and Optimization" (2016). Theses and Dissertations. 1748.