Date of Graduation

12-2016

Document Type

Dissertation

Degree Name

Doctor of Philosophy in Engineering (PhD)

Degree Level

Graduate

Department

Chemical Engineering

Advisor

Xianghong Qian

Committee Member

Jeff Wolchok

Second Committee Member

Peter Czermak

Third Committee Member

Steve Cramer

Fourth Committee Member

Ranil Wickramasinghe

Keywords

Pure sciences; Applied sciences; ATRP; Biologics; Downstream process; Membrane chromatography; Protein separations; Surface modification

Abstract

Membrane chromatography, or membrane adsorber, represents an attractive alternative to conventional packed bed chromatography used in downstream processing. Membrane chromatography has many advantages, including high productivity, low buffer consumption and ease to scale up. This doctoral dissertation focuses on developing novel polymeric ligands for protein separations using membrane chromatography. Atom transfer radical polymerization (ATRP), known as a controlled radical polymerization technique, has been used to control the architecture of grafted polymeric ligands. The center theme of this dissertation is to develop new polymeric ligands and investigate how the polymer’s property (e.g. flexibility, hydrophobicity) and architecture (e.g. chain density, chain length) affect the protein separation performances.

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