Date of Graduation
Master of Science in Biomedical Engineering (MSBME)
Second Committee Member
Amylin, Capillary Electrophoresis, hIAPP, Protein Aggregation
Amylin (hIAPP) aggregates have been found in 90% of patients with type II diabetes at autopsy, and are suspected to play a role in the death of islet &beta-cells1. However, this aggregation process is not well understood. Here, we explore methods that utilize capillary electrophoresis (CE) as a means to better understand amylin's aggregation process.
We examined the effects of solutions conditions: agitation, pH, salt, and temperature on amylin aggregation using Thioflavin T, dot blots, and capillary electrophoresis. Thiofalvin T was used to predict the lag time to &beta-sheet formation. Our results indicated all variables with the exception of agitation were feasible for study with CE. Capillary electrophoresis was then employed to observe the formation of oligomers with dot blots used as a confirmation technique. Overall, results showed all solutions conditions examined promote aggregation, however there was variance between time courses. Conflicting results suggest further study is needed to fully understand the observed amylin aggregation phenomena.
May, Michael, "ThT and Capillary Electrophoresis to Monitor the Effects of Solutions Conditions on Amylin Aggregation" (2014). Theses and Dissertations. 2311.