Date of Graduation

8-2017

Document Type

Thesis

Degree Name

Master of Science in Chemical Engineering (MSChE)

Degree Level

Graduate

Department

Chemical Engineering

Advisor

Robert R. Beitle

Committee Member

Lauren Greenlee

Second Committee Member

Roger Koeppe

Third Committee Member

Nicholas Bedford

Keywords

Fed Batch Fermentation, Metal Binding Peptide, Nanoparticle, Recombinant Production

Abstract

There is a need for low-cost nanoparticle materials in the context of new technologies for catalyst development. The purpose of this work was to recombinantly produce a 45- amino acid long metal binding peptide that is useful for nanoparticle synthesis. Using splicing by overlap extension PCR, a synthetic gene containing the fusion of the metal binding peptide with Green Fluorescent Protein (GFPUV) was constructed. The metal binding peptide, fused to reporter protein GFPUV, was expressed using high cell density fermentation. Palladium nanoparticles of an average diameter of 1.18 0.45 nm were synthesized by using the crude cell extract containing the fusion protein. Nanoparticle synthesis was also done using desalted samples (removal of medium components) as well as enriched fractions from ion exchange chromatography purification. In all cases, palladium, gold and palladium-gold nanoparticles were successfully synthesized with good particle size for catalysis applications, control of diameter, and lack of other metal precipitants, respectively.

This work illustrates that metallic nanoparticles can be synthesized using the soluble cell extract containing the fusion protein without extensive purification or cleavage steps.

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