Date of Graduation

5-2018

Document Type

Dissertation

Degree Name

Doctor of Philosophy in Cell & Molecular Biology (PhD)

Degree Level

Graduate

Department

Biological Sciences

Advisor/Mentor

Steven C. Ricke

Committee Member

Ravi D. Barabote

Second Committee Member

Bennie J. Bench

Third Committee Member

Young Min Kwon

Fourth Committee Member

Jeffrey A. Lewis

Keywords

Microbiome, Next generation sequencing, Poultry, Pseudomonas, slaughter

Abstract

The United States poultry industry generated 38.6 billion pounds (17,500 metric ton) of meat in 2014 which averaged to 121 pounds (55 kg) per individual of the U.S that same year. Of that meat generated by the poultry industry, an estimated 1 million cases of Salmonellosis will occur. Out of the 1 million cases approximately 40, 000 to 50,000 will be confirmed cases by the CDC. Recently, the USDA has requested changes in the inspection process and are currently allowing processors more freedom to utilize innovation to drive the increase in safer and more desirable foods. The new standards set forth by the USDA and the willingness to be more flexible with processors will create an atmosphere conducive for the development of new technologies, process design, and antimicrobial intervention strategies that are synergistic with the rate at which large scale production occurs. In this review, the production process will be explored in conjunction with the regulatory statutes that govern poultry slaughter. Additionally, the mechanism in which antimicrobials interact with bacteria and the employment of Next Generation Sequencing to gain better insight of how the intervention strategies decontaminate raw meat.

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