Date of Graduation
8-2012
Document Type
Thesis
Degree Name
Master of Science in Poultry Science (MS)
Degree Level
Graduate
Department
Poultry Science
Advisor/Mentor
Walter G. Bottje
Committee Member
Charles Maxwell
Second Committee Member
Robert Wideman
Third Committee Member
Billy Hargis
Fourth Committee Member
Byung-Whi Kong
Keywords
Biological sciences, Hepatoma, Oxidative stress, Poultry
Abstract
Bioenergetic mechanisms responsible for ATP production are essential in carrying out maintenance and cell-specific functions. In this study, hepatocytes (liver cells) were used to test both endogenous and exogenous stress on cellular respiration. The secondary lipid peroxide, 4-hydroxynonenal (HNE), was used because it alters bioenergetics by increasing mitochondrial proton leak that attenuates mitochondrial radical production and, therefore, endogenous oxidative stress. The major objective of this study was to demonstrate effects of HNE-induced oxidative stress on avian hepatocyte bioenergetics. Various chemical which help enable the determination of oxygen (O2) consumption linked to ATP synthesis (oligomycin), maximal O2 consumption (FCCP), and proton leak (antimycin a) were used during bioenergetic analysis. Results indicated that both hepatocyte cell lines used exhibited increases in maximal O2 consumption in response to 5 and 10 ìM HNE, whereas 30 ìM HNE enhanced proton leak.
Citation
Piekarski, A. L. (2012). Cell Bioenergetics in Leghorn Male Hepatoma Cells and Immortalized Chicken Liver Cells in Response to 4-Hydroxynonenal Induced Oxidative Stress. Graduate Theses and Dissertations Retrieved from https://scholarworks.uark.edu/etd/484
Included in
Cell Biology Commons, Organismal Biological Physiology Commons, Poultry or Avian Science Commons