Date of Graduation
5-2013
Document Type
Thesis
Degree Name
Master of Science in Food Science (MS)
Degree Level
Graduate
Department
Food Science
Advisor/Mentor
Luke Howard
Committee Member
Andy Proctor
Second Committee Member
Neil Allison
Keywords
Biological sciences, Extraction, Flash chromatography, Food science, Hplc analysis, Tocotrienol, Vitamin e
Abstract
The tocols (alpha-, beta-, gamma- and delta- tocopherols (T); alpha-, beta-, gamma- and delta-tocotrienols (T3)) make up the vitamin E family of antioxidants. Tocols have many health benefits. Gamma-T3 has been shown to protect against harmful radiation effects in mice. During rice bran oil refining up to 90% of tocols in rice bran are lost to the deodorizer distillate. The objective of this study was to optimize parameters for analyzing and isolating tocols from rice bran oil deodorizer distillate (RBODD). NP-HPLC analysis of tocol standards was optimized by testing ten mobile phases. Tocol extraction was optimized by testing three concentration solvents (acetonitrile, methanol, ethanol) using various solvent:RBODD ratios (5:1, 10:1, 15:1, 20:1) at two temperatures (4°C, -20°C). Hexane (HX)-ethyl acetate (EA)-acetic acid (AA) (97.3:1.8:0.9 v/v/v) provided baseline resolution of all eight tocols. All interactions including solvent*ratio*temperature had a significant effect on tocol levels in the extracts (p
Citation
Kordsmeier, M. (2013). Extraction and Purification of Gamma-Tocotrienol from Rice Bran Oil Deodorizer Distillate. Graduate Theses and Dissertations Retrieved from https://scholarworks.uark.edu/etd/705