Date of Graduation

5-2013

Document Type

Thesis

Degree Name

Master of Science in Microelectronics-Photonics (MS)

Degree Level

Graduate

Department

Graduate School

Advisor

Ken Vickers

Committee Member

Paul David

Second Committee Member

Rick Ulrich

Keywords

Pure sciences; Microdialysis; Recycled flow

Abstract

Incomplete recovery during microdialysis sampling is hindering important research in neurology, proteomics, and immunology. Although the current generalized solution, decreasing volumetric flow rates (Q), has been and will remain to be a useful strategy it has reached it's a physical limitation due to evaporation at the collection site. Consequently, many important signaling molecules, such as signaling proteins, remain difficult to study. It is more fundamental to consider relative recovery as a function of the interaction time between the perfusate and the environment surrounding the probe.

In this work an increase in relative recovery was predicted by a mathematical model. Using recycled flow and flow reversal an increase in extraction efficiency was achieved at constant Q. It was observed that the recovery increase decrease as the number of passes increase.

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