Date of Graduation
Master of Science in Microelectronics-Photonics (MS)
Second Committee Member
Incomplete recovery during microdialysis sampling is hindering important research in neurology, proteomics, and immunology. Although the current generalized solution, decreasing volumetric flow rates (Q), has been and will remain to be a useful strategy it has reached it's a physical limitation due to evaporation at the collection site. Consequently, many important signaling molecules, such as signaling proteins, remain difficult to study. It is more fundamental to consider relative recovery as a function of the interaction time between the perfusate and the environment surrounding the probe.
In this work an increase in relative recovery was predicted by a mathematical model. Using recycled flow and flow reversal an increase in extraction efficiency was achieved at constant Q. It was observed that the recovery increase decrease as the number of passes increase.
Deaton, Justin Cole, "Improvements in Microdialysis Sampling Extraction Efficiency Using Recycled Flow" (2013). Theses and Dissertations. 814.