Date of Graduation
Bachelor of Science in Agricultural, Food and Life Sciences
In 2013, Castro et al., produced the first linkage map for primocane-fruiting blackberries using the cross ‘Prime-Jim®’ x ‘Arapaho.’ This mapping population has been maintained since the original experiment with the hopes of conducting future studies. Further research was determined to be needed on the population to better characterize additional phenotypic traits, and since nine years had passed since the seedlings were established, there was a need to assess the continued genetic purity of the population using molecular methods. Phenotypic data was collected to analyze the variation of the population including soluble solids content, titratable acidity, average weight, shape, and firmness. While the population showed significant variation for each quantitative trait measured, the molecular results showed that none of the evaluated progeny matched the marker banding patterns expected based on previous results. Thus, the population was determined to be mislabeled or possibly some plants had grown together contributing to the impurity found. I recommended starting over with a new cross for future molecular mapping studies.
Luther, Loren M., "Phenotypic Variation and Genetic Purity of the Original 'Prime-Jim®' x 'Arapaho' Population" (2017). Horticulture Undergraduate Honors Theses. 5.
Agricultural Science Commons, Botany Commons, Fruit Science Commons, Genetics Commons, Horticulture Commons, Molecular Genetics Commons, Other Plant Sciences Commons, Plant Biology Commons, Plant Breeding and Genetics Commons, Plant Pathology Commons