Serum from three species of water snakes (Nerodia rhombifera, N. erythrogaster and N. fasciata) from one geographic region was analyzed electrophoretically on cellulose acetate, and anodic mobility and relative concentration of the fractions were determined by a recording densitometer with an automatic integrator. Classification of fractions was based on mobility (Rf, values), and for identification purposes, bands were labeled in order of decreasing mobility (albumin and alpha₁, alpha₂, alpha₃, beta₁, beta₂, gamma₁, and gamma₂ globulins). Seven fractions were identified in each species with alpha₃ being absent from N. rhombifera and N. erythrogaster, and only one gamma fraction was observed in N. fasciata. In the three species, gamma globulin was the predominant protein (42-46%), and albumin levels were characteristically low ;however, a distinct difference was observed in albumin concentration (N. fasciata, 7%; N. rhombifera and N. erythrogaster, 16-18%). The Rf values and relative concentrations of other globulins showed heterogeneity in the three species, with the protein pattern of N. fasciata being distinct from the other two species.
Garnett, Phyllis J.
"Electrophoretic Analysis of Blood Serum Proteins in Three Species of Water Snakes (Genus Nerodia),"
Journal of the Arkansas Academy of Science: Vol. 33
, Article 13.
Available at: http://scholarworks.uark.edu/jaas/vol33/iss1/13