The influence of cold (4°C) used to prepare cells for metabolic investigations was examined with Trichomonas gallinae in this study. Cells washed with cold diluent in a refrigerated centrifuge were found to be less stimulated in their gas production at 37°C when exposed to glucose or maltose than cells washed at room temperature conditions. Such cold-prepared cells had higher initial glycogen contents after washing, faster endogenous glycogen degradation rates when incubated at 37 °C, but lower glycogen synthesis in the presence of glucose or maltose when compared to cells not prepared in the cold. However, uptake of glucose and maltose at 37 °C was not affected by pretreatment with cold. Washing with cold also reduced the total number of recoverable cells by an average of 20%. Cold washing of T. gallinae in three diluents (modified Ringers, Krebs Ringer phosphate, and 2% Trypticase) increased the recovery or lag time in STS medium when compared to use of the three washing diluents at room temperature.
Daly, James J.
"Effect of Cold Shock on the Metabolism of Trichomonas Gallinae,"
Journal of the Arkansas Academy of Science: Vol. 34
, Article 14.
Available at: http://scholarworks.uark.edu/jaas/vol34/iss1/14