Efficient regeneration of in vitro cultures of Oryza sativa L.is essential for successful manipulation of recombinant DNA technologies. Arkansas rice varieties perform better on modified Gamborg (B5)media, than on Murashige and Skoog (MS) orN6 media, which are more frequently reported in the literature. While 2,4-dichlorophenoxyacetic acid (2,4-D) is commonly used for regeneration treatments of rice, Picloram (Pic) provides a possible alternative as a synthetic auxin source. Although regeneration frequency appears low, complete regeneration (whole plantlets) was faster and development of the shoot was superior on picloram treatments as compared to 2,4-D. Screening 5 Arkansas varieties for regeneration efficiency on 3 treatments, 2 drawn from the literature and 1 based on Pic, shows distinct rankings for successful identification of totipotent established lines. LaGrue, important for both production and breeding purposes, shows the highest ranking for successful regeneration as well as for uniform response of individual seedling lines across regeneration treatments. Other varieties proved unsuitable for transformation work due to lack of regeneration response and/or non-uniform response to regeneration treatments. Identifying these parameters will facilitate the ultimate transfer of recombinant DNA into tropical Japonica rice types grown in Arkansas for trait improvement or gene expression studies.
Chowdhury, Moytri Roy; Dabul, Audrei; Hubstenburger, John; and Phillips, Gregory
"Development of in vitro Regeneration Protocols for Arkansas Rice Varieties (Oryza sativa L.),"
Journal of the Arkansas Academy of Science: Vol. 60
, Article 17.
Available at: http://scholarworks.uark.edu/jaas/vol60/iss1/17