Document Type

Patent

Publication Date

1-6-2015

Abstract

Provided is a separatome-based recombinant peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome, such as that of E. coli, yeast, Bacillus subtilis or other prokaryotes, insect cells, mammalian cells, etc. This platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both, with emphasis on the former. Moreover, the platform provides a computerized knowledge tool that, given separatome data and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies leading to efficient product purification. The separatome-based protein expression and purification platform is an efficient bioseparation system that intertwines host cell expression systems and chromatography.

Department

Biological Sciences; Chemical Engineering

Patent Number

US8927231

Application Number

US20140065672

Application Published

3-6-2014

Application Filed

10-17-2013

Assignee

Board of Trustees of the University of Arkansas (Little Rock, AR); University of Pittsburgh--Of The Commonwealth System of Higher Education (Pittsburgh, PA)

Comments

Ellen M. Brune, Department of Chemical Engineering
Robert Beitle, Jr., Department of Chemical Engineering
Ralph Henry, Department of Biological Sciences

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