Provides a method of forming recipient oocytes. The method generally include taking a cumulus-free oocytes, and separating the chromosomal material from the oocyte by centrifuging against a density gradient material to force the chromosomal material to pass through the cytoplasmic membrane. A method of cloning is also describe that fuses a recipient oocyte with a donor cell.
Board of Trustees of the University of Arkansas (Little Rock, AR)
Rosenkrans, Charles F. Jr. and Wagoner, Eddie J., "Functional enucleation of bovine oocytes" (1999). Patents Granted. 186.