Date of Graduation
Bachelor of Science
My research project examines how the deletion of the cytoplasmic domain affects the function of Robo3. If Robo3 is signaling repulsion in response to SLIT this activity should require the cytoplasmic domain. I investigated the functional importance of Robo3 by deleting the cytoplasmic domain of Robo3 using a CRISPR-based technique. This modified gene was then injected into Drosophila embryos where it replaced the normal copy of the gene. Embryos expressing the modified version of robo3 in place of normal robo3 were dissected and examined.
Cytoplasmic domain, Robo3, CRISPR, gene modification, embryos
Agcaoili, J. (2022). The Effects of Deletion of the Cytoplasmic Domain of Robo3 on Drosophila. Biological Sciences Undergraduate Honors Theses Retrieved from https://scholarworks.uark.edu/biscuht/60
Biology Commons, Molecular and Cellular Neuroscience Commons, Molecular Genetics Commons