The Effects of Deletion of the Cytoplasmic Domain of Robo3 on Drosophila

Author

Jessie AgcaoiliFollow

Date of Graduation

5-2022

Document Type

Thesis

Degree Name

Bachelor of Science

Degree Level

Undergraduate

Department

Biological Sciences

Advisor/Mentor

Evans, Tim

Abstract

My research project examines how the deletion of the cytoplasmic domain affects the function of Robo3. If Robo3 is signaling repulsion in response to SLIT this activity should require the cytoplasmic domain. I investigated the functional importance of Robo3 by deleting the cytoplasmic domain of Robo3 using a CRISPR-based technique. This modified gene was then injected into Drosophila embryos where it replaced the normal copy of the gene. Embryos expressing the modified version of robo3 in place of normal robo3 were dissected and examined.

Keywords

Cytoplasmic domain, Robo3, CRISPR, gene modification, embryos

Citation

Agcaoili, J. (2022). The Effects of Deletion of the Cytoplasmic Domain of Robo3 on Drosophila. Biological Sciences Undergraduate Honors Theses Retrieved from https://scholarworks.uark.edu/biscuht/60