Document Type
Article
Publication Date
11-2024
Keywords
diffuse reflectance spectroscopy; joint infection; scattering; synovial fluid
Abstract
Severe joint infections, such as septic arthritis, require rapid diagnostic testing of the synovial fluid aspirated from joints level so that a surgical team can be assembled quickly. We present a diffuse reflectance spectroscopy (DRS) system for noncontact determination of infection. Using a light-tight syringe holder and fiber optic probe, diffusely reflected light from 475 to 655 nm was acquired from 18 patient samples through the wall of a syringe in a noncontact and sterile manner. We determined the reflectance ratios at two different wavelengths—R 490/R 600 and R 580/R 600 and found statistically significant differences (p < 0.05) in both ratios between the infected and noninfected groups. Critically, the R 490/R 600 and R 580/R 600 ratios were significantly correlated with clinical biomarkers—the white blood cell (WBC) and red blood cell (RBC) counts, respectively. This study demonstrates the potential of DRS as a rapid diagnostic tool for joint infections.
Citation
Drewke, E. E., Brand, R. L., Geels, C. G., Jensen, H. K., Wong, K., Sanders, J. D., & Rajaram, N. (2024). Noncontact Diffuse Reflectance Spectroscopy of Synovial Fluid Samples for Rapid Identification of Infections. Journal of Biophotonics, 17 (11) https://doi.org/10.1002/jbio.202400213
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
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Biochemistry Commons, Biomedical Engineering and Bioengineering Commons, Biophysics Commons, Optics Commons
