Date of Graduation


Document Type


Degree Name

Bachelor of Science in Biomedical Engineering

Degree Level



Biomedical Engineering


Rajaram, Narasimhan

Committee Member/Third Reader

Quinn, Kyle

Committee Member/Fourth Reader

Rao, Raj


Recently, there has been a larger use of 2-NBDG, a fluorescent glucose analog, to study glucose uptake in different cell types. These cell types have ranged anywhere from bacteria to human cancer cells. However, there has yet to be a standard procedure and practice for using 2-NBDG. In this study, our goal is to create a standard fasting time for the cells before introducing 2-NBDG to them. This study uses 4T07 cells, a murine breast cancer cell line, to help optimize this fasting time. These cells were fasted at different time points in order to find the ideal fasting time. This ideal fasting time ensures the highest uptake of 2-NBDG in the cells. Intensity measurements, resulting from 2-NBDG fluorescence, were calculated to determine the optimal fasting time. The following paper details the procedure used including cell culturing and passaging, microscope setup, fluorescent imaging, and image processing.