Date of Graduation

5-2025

Document Type

Thesis

Degree Name

Bachelor of Science in Chemistry

Degree Level

Undergraduate

Department

Chemistry & Biochemistry

Advisor/Mentor

Dong, Bin

Committee Member

Stenken, Julie

Second Committee Member

Vyas, Reeta

Third Committee Member

Chen, Jingyi

Abstract

This thesis is focused on recent advancements made in the field of chemical imaging, optical microscopy, and recent developments that have improved spatial, temporal, or spectral resolution or have made certain techniques more accessible and cost effective for their wider adoption. This thesis is organized based on the systems of interest we studied with each new technique. Although, it is important to note that many of the methods here can be used with a multitude of systems even if not explicitly mentioned. The first chapter of this thesis will discuss the current state of chemical imaging, common terminology, and important first notes to assist the reader in more complex future chapters. Chapter7 two focuses on our recent advancements in spectroscopically enabled microscopy with singleparticle specificity with implications across many disciplines to study a variety of systems. Chapter three discusses advancements in five-dimensional single particle orientation and rotational tracking (SPORT) for two main purposes: monitoring of real-time biological processes and empirical single particle diffusion observed in accordance with the Stokes-Einstein relation. Chapter four will discuss bioinspired self-organizing lattices of various nanomaterials that have potential applications as highly sensitive detectors along with the analysis of these lattices by grazing incident small-angle x-ray scattering (GISAXS) and x-ray reflectivity (XRR). Chapter five will focus on our advances in single-molecule localization microscopy (SMLM) for superresolution imaging by direct stochastic optical reconstruction microscopy (dSTORM) with true color single molecule spectral in the analysis of biological nanostructures.

Keywords

Super-resolution; SMLM; Chemical Imaging; Single-particle Tracking; Fluorescence Microscopy; GISAXS

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