Date of Graduation

12-2019

Document Type

Dissertation

Degree Name

Doctor of Philosophy in Chemistry (PhD)

Degree Level

Graduate

Department

Chemistry & Biochemistry

Advisor

Colin Heyes

Committee Member

Paul Adams

Second Committee Member

Jingyi Chen

Third Committee Member

Ralph Henry

Fourth Committee Member

Suresh Thallapuranam

Keywords

cpSRP, disordered, dynamics, protein, structure, targeting

Abstract

The work presented in this dissertation explores the structural dynamics in the chloroplast signal recognition particle pathway. Findings include cpSRP shows scanning functionality similar to that in the cytosolic SRP with the ribosome. The intrinsically disordered C-terminal tail of the Albino3 protein has some transient secondary structure. Upon binding to cpSRP43 in solution, separate secondary structure formation was identified in the C-terminal tail of Albino3. Finally, to increase efficiency of analyzing fluorescence time traces for this work, a modular software was produced.

Available for download on Saturday, December 19, 2020

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