Date of Graduation
Doctor of Philosophy in Biology (PhD)
Douglas D. Rhoads
Steven J. Beaupre
Second Committee Member
David M. Ivey
Third Committee Member
Ascites, Crotalus horridus, Gene flow, Genotype, Pulmonary hypertension, Variable number tandem repeats
We used variable number tandem repeats (VNTR) to validate the chicken as a human medical model for Pulmonary Arterial Hypertension. We identified seven regions on four chromosomes and interrogated for VNTR markers that significantly associate with Pulmonary Hypertension Syndrome/ascites. In those regions, we identified 7 candidate genes; AGTR1, ACE, p38MAPK, SST, 5HT2B, NET1, and CALM3 for further analysis as significantly contributing QTL for ascites/PHS. We also used variable number tandem repeats to measure gene flow and gather evidence for multiple paternity in a population of Timber rattlesnakes, Crotalus horridus. We were able to verify 1 VNTR that can be used to assess gene flow in and among our populations. We were also able to use this VNTR to provide evidence of multiple paternity in the Timber rattlesnake.
Smith, Candace D., "Applications of Variable Number Tandem Repeat Genotyping in the Validation of an Animal Medical Model and Gene Flow Studies in Threatened Populations of Reptiles" (2009). Theses and Dissertations. 49.