Two hundred forty-two specimens of Onychomys leucogaster, ranging in age from day of birth to the twenty-eighth day were cleared and stained using both Alizarin KOH and Alcian Blue/Alizarin-Trypsin staining methods. Thirty centers of ossification were studied. The data demonstrate the following: 1) skeletal centers of the appendicular skeleton ossify and mature earliest; 2) a new sesamoid bone lateral to the distal condyles of the femur was discovered; 3) the skeletal ossification of the baculum is present at one day of age; 4) a high degree of individual variation precludes aging of this species by skeletal maturation.

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