A biosensor is an analytical device that uses biomaterials as elements of the sensing system and converts a biological response into an electrical signal. Biodiesel is a bio-based alternative, biodegradable, renewable, nontoxic diesel fuel made from a chemical reaction between alcohol (usually methanol or ethanol) and plant oil or animal fat. A need to provide accurate, real-time information for the quality sensing of biodiesel properties such as free and total glycerol has led to an ever-increasing demand for biosensor development. Being able to monitor specific physical and chemical properties is the prerequisite for developing a biosensor for quality sensing of the biodiesel. This article proposes a method for detection of the blend level of degraded biodiesel and lipase as a bioelement of biosensor systems. A design of an electrochemical potentiometric biosensor for quality sensing of biodiesel properties is proposed and discussed in detail. However, experimental trials, actual implementation and evaluations are necessary to understand the feasibility of the proposed biodiesel biosensor.

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