Document Type

Patent

Publication Date

5-7-2019

Abstract

In one aspect, affinity tags for recombinant protein purification are described herein which, in some embodiments, can mitigate or overcome disadvantages of prior affinity tag systems. In some embodiments, for example, affinity tags described herein permit efficient elution of desired recombinant proteins with simplified solution systems, such as alkali metal salt solutions. An affinity tag described herein comprises an amino acid sequence including a repeating amino acid unit of BXXXBXX, wherein B is an amino acid selected from the group consisting of histidine, lysine and arginine and X is an amino acid selected from the group consisting of amino acids other than histidine, lysine and arginine.

Department

Biological Sciences; Chemistry and Biochemistry

Patent Number

US10280196

Application Number

US 20170029461 A1

Application Published

2-2-2017

Application Filed

1-20-2015

Assignee

Board of Trustees of the University of Arkansas (Fayetteville, AR)

Comments

Thallapuranam Krishnaswamy Suresh Kumar, Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR
Srinivas Jayanthi, Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR
Jacqueline Morris, Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR
Alicia D. Brown, Department of Biological Sciences, University of Arkansas, Fayetteville, AR
David S. McNabb, Department of Biological Sciences, University of Arkansas, Fayetteville, AR
Ralph Henry, Department of Biological Sciences, University of Arkansas, Fayetteville, AR

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