Document Type
Patent
Publication Date
5-7-2019
Abstract
In one aspect, affinity tags for recombinant protein purification are described herein which, in some embodiments, can mitigate or overcome disadvantages of prior affinity tag systems. In some embodiments, for example, affinity tags described herein permit efficient elution of desired recombinant proteins with simplified solution systems, such as alkali metal salt solutions. An affinity tag described herein comprises an amino acid sequence including a repeating amino acid unit of BXXXBXX, wherein B is an amino acid selected from the group consisting of histidine, lysine and arginine and X is an amino acid selected from the group consisting of amino acids other than histidine, lysine and arginine.
Department
Biological Sciences; Chemistry & Biochemistry
Patent Number
US10280196
Application Number
US 20170029461 A1
Application Published
2-2-2017
Application Filed
1-20-2015
Assignee
Board of Trustees of the University of Arkansas (Fayetteville, AR)
Citation
Kumar, T., Jayanthi, S., Morris, J., Brown, A. D., McNabb, D. S., & Henry, R. (2019). Heparin affinity tag and application thereof. Patents Granted. Retrieved from https://scholarworks.uark.edu/pat/363
Comments
Thallapuranam Krishnaswamy Suresh Kumar, Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR
Srinivas Jayanthi, Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR
Jacqueline Morris, Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR
Alicia D. Brown, Department of Biological Sciences, University of Arkansas, Fayetteville, AR
David S. McNabb, Department of Biological Sciences, University of Arkansas, Fayetteville, AR
Ralph Henry, Department of Biological Sciences, University of Arkansas, Fayetteville, AR