Document Type

Patent

Publication Date

7-20-2021

Abstract

Methods of gene stacking are described herein. The methods can be used to repeatedly add genes into a chosen locus in a precise manner, which ensures co-segregation of all introduced genes and contributes to the stabilization of gene expression. In addition, methods of removing any additional foreign DNA elements such as selectable markers are provided. Seed stocks or cell lines comprising a gene stacking site, vectors containing an insert flanked by target sites for a site-specific DNA recombinase for use in the methods and kits for carrying out the methods are also provided herein.

Department

Crop, Soil and Environmental Sciences

Patent Number

US11066676

Application Number

US 20190323021 A1

Application Published

10-24-2019

Application Filed

3-1-2019

Assignee

Board of Trustees of the University of Arkansas (Little Rock, AR)

Comments

Vibha Srivastava, Department of Crop, Soil and Environmental Sciences, University of Arkansas, Fayetteville, AR

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