Assessment of the Impact of Humic Acids on Intestinal Microbiota, Gut Integrity, Ileum Morphometry, and Cellular Immunity of Turkey Poults Fed an Aflatoxin B₁-Contaminated Diet

Authors

Jesus A. Maguey-Gonzalez, University of Arkansas, FayettevilleFollow
Jing Liu, Oklahoma State UniversityFollow
Guolong Zhang, Oklahoma State UniversityFollow
Juan D. Latorre, University of Arkansas, FayettevilleFollow
Juan O. Hernandez-Ramirez, National Autonomous University of MexicoFollow
Maria de Jesus Nava-Ramirez, National Autonomous University of MexicoFollow
Roberto Señas-Cuesta, University of Arkansas, FayettevilleFollow
Sergio Gomez-Rosales, Centro Nacional de Investigación Disciplinaria en Fisiología y Mejoramiento AnimalFollow
Maria de Lourdes Angeles, Centro Nacional de Investigación Disciplinaria en Fisiología y Mejoramiento AnimalFollow
Andressa Stein, University of Arkansas, FayettevilleFollow
Bruno Solis-Cruz, Universidad Nacional Autonoma de MexicoFollow
Daniel Hernandez-Patlan, Universidad Nacional Autonoma de MexicoFollow
Ruben Merino-Guzman, Universidad Nacional Autonoma de MexicoFollow
Xochitl Hernandez-Velasco, National Autonomous University of MexicoFollow
Inkar Castellanos-Huerta, University of Arkansas, FayettevilleFollow
Santiago Uribe-Diaz, University of Arkansas, FayettevilleFollow
Alma Vazquez-Duran, National Autonomous University of MexicoFollow
Abraham Mendez-Albores, National Autonomous University of MexicoFollow
Victor Petrone-Garcia, National Autonomous University of MexicoFollow
Guillermo Tellez Jr., University of Arkansas, FayettevilleFollow
Billy M. Hargis, University of Arkansas, FayettevilleFollow
Guillermo Tellez-Isaias, University of Arkansas, FayettevilleFollow

Document Type

Article

Publication Date

3-2024

Keywords

turkey poults; aflatoxin B-1; humic acids; intestinal microbiota; gut integrity; morphometric studies

Abstract

A recent study published data on the growth performance, relative weights of the organs of the gastrointestinal tract, liver histology, serum biochemistry, and hematological parameters for turkey poults fed an experimental diet contaminated with aflatoxin B₁ (AFB₁) and humic acids (HA) extracted from vermicompost. The negative effects of AFB₁ (250 ng AFB₁/g of feed) were significantly reduced by HA supplementation (0.25% w/w), suggesting that HA might be utilized to ameliorate the negative impact of AFB₁ from contaminated diets. The present study shows the results of the remaining variables, as an extension of a previously published work which aimed to evaluate the impact of HA on the intestinal microbiota, gut integrity, ileum morphometry, and cellular immunity of turkey poults fed an AFB₁-contaminated diet. For this objective, five equal groups of 1-day-old female Nicholas-700 turkey poults were randomly assigned to the following treatments: negative control (basal diet), positive control (basal diet + 250 ng AFB₁/g), HA (basal diet + 0.25% HA), HA + AFB₁ (basal diet + 0.25% HA + 250 ng AFB₁/g), and Zeolite (basal diet + 0.25% zeolite + 250 ng AFB₁/g). In the experiment, seven replicates of ten poults each were used per treatment (n = 70). In general, HA supplementation with or without the presence of AFB₁ showed a significant increase (p < 0.05) in the number of beneficial butyric acid producers, ileum villi height, and ileum total area, and a significant reduction in serum levels of fluorescein isothiocyanate–dextran (FITC-d), a marker of intestinal integrity. In contrast, poults fed with AFB₁ showed a significant increase in Proteobacteria and lower numbers of beneficial bacteria, clearly suggesting gut dysbacteriosis. Moreover, poults supplemented with AFB₁ displayed the lowest morphometric parameters and the highest intestinal permeability. Furthermore, poults in the negative and positive control treatments had the lowest cutaneous basophil hypersensitivity response. These findings suggest that HA supplementation enhanced intestinal integrity (shape and permeability), cellular immune response, and healthier gut microbiota composition, even in the presence of dietary exposure to AFB₁. These results complement those of the previously published study, suggesting that HA may be a viable dietary intervention to improve gut health and immunity in turkey poults during aflatoxicosis.

Citation

Maguey-Gonzalez, J. A., Liu, J., Zhang, G., Latorre, J. D., Hernandez-Ramirez, J. O., Nava-Ramirez, M., Señas-Cuesta, R., Gomez-Rosales, S., Angeles, M., Stein, A., Solis-Cruz, B., Hernandez-Patlan, D., Merino-Guzman, R., Hernandez-Velasco, X., Castellanos-Huerta, I., Uribe-Diaz, S., Vazquez-Duran, A., Mendez-Albores, A., Petrone-Garcia, V., Tellez, G., Hargis, B. M., & Tellez-Isaias, G. (2024). Assessment of the Impact of Humic Acids on Intestinal Microbiota, Gut Integrity, Ileum Morphometry, and Cellular Immunity of Turkey Poults Fed an Aflatoxin B₁-Contaminated Diet. Toxins, 16 (3), 122. https://doi.org/10.3390/toxins16030122

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This work is licensed under a Creative Commons Attribution 4.0 International License.