Thrombin-like activities were not observed inCrotalus basiliscus, C. molossus and C. scutulatus scutulatus crude venoms. 5'-Nucleotidase specific activities of 0.863, 0.273 and 5.520 units/mg of crude venom protein were observed inC. basiliscus, C. molossus and C. s. scutulatus venoms, respectively. Concanavalin ASepharose 4 B (Con A)affinitychromatography yielded two fractions from each of the crude venoms. Ineach instance, both fractions exhibited 5'-nucleotidase activities and the Con A-binding proteins had higher activities than the Con A-nonbinding proteins. 5'-Nucleotidase activities inthe DEAESephadex A-50 chromatographic fractions were localized in the first elution fraction and the last fraction(s) to elute. EDTAhad no effect on the 5'-nucleotidase activities ofthe crude venoms.
Shams, Ali M.S.; Sifford, Dewey H.; and Johnson, Bob D.
"5'-Nucleotidase and Thrombin-Like Activities of Selected Crotalid Venoms,"
Journal of the Arkansas Academy of Science: Vol. 49
, Article 36.
Available at: http://scholarworks.uark.edu/jaas/vol49/iss1/36