Precursor incorporation into uterine lipids was examined in vitro after estradiol-17ß administration in immature female rats. The effect of adding supplemental substrates, glucose or gluconate, to the incubation medium on the labeling pattern of pyruvate-3-¹⁴C into uterine lipids was studied. Presence of supplemental substrate in the medium enhanced the incorporation of pyruvate into uterine lipids after two hours of in vivo estrogen treatment. It is suggested that estrogen's acceleration of pyruvate incorporation into lipid may be due to a concomitant effect on glucose metabolism.

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