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Abstract

Tissue culture methods have been established to regenerate certain rice (Oryza sativa L) cultivars, but regeneration of the rice cultivars widely grown in Arkansas has not been reported. This study has established an in vitroculture for the rice cultivars 'Nortai', 'Starbonnet', 'Mars', Tebonnet', 'Newbonnet', and 'Lemont'. Callus was induced in the dark at either 20 or 28 C from dehusked seeds cultured on Murashige and Skoog (MS) medium (Murashige and Skoog, 1962) containing 40 g L^-1 sucrose, 10 g L^1 agar, 0.5, 1.0, or 2.0 mg L^-1 1 2,4-dichlorophenoxyacetic acid (2,4-D) and adjusted to pH 5.7. After four weeks the calli were weighed, transferred onto MS medium containing no 2,4-D, and maintained in a 1 2-h photoperiod (65 uE m^-2 s^-1) at 25 ± 2 C to induce plant regeneration. Callus production was best when cultured on a medium containing 1.0 mg L^-1 2,4-D and incubated at 28 C. Plant regeneration was observed two to four weeks later. The percentage of calli regenerating platlets varied with the cultivar and the callus induction treatment. Callus induction at 20 C on a medium with a 2,4-D level less than 2.0 mg L^-1 enhanced the regenerability of most cultivars. Regenerates were transplanted to soil and grow normally to maturity. This system can be helpful in improving rice cultivars with tissue culture techniques such as somaclonal variant selection and somatic hybridization.

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