Date of Graduation

5-2016

Document Type

Thesis

Degree Name

Bachelor of Science

Degree Level

Undergraduate

Department

Chemistry & Biochemistry

Advisor/Mentor

Stenken, Julie

Committee Member/Reader

Paul, David

Committee Member/Second Reader

Barabote, Ravi

Committee Member/Third Reader

Suresh, Kumar

Abstract

Microdialysis is a sampling technique that is used to collect analytes through a semipermeable membrane. The collection occurs via the microdialysis probe where analytes can diffuse through the membrane and be collected by use of the outlet. Microdialysis is typically utilized with solutes that are hydrophilic and have a low molecular weight. It is unusual for hydrophobic solutes to be used, but pentane, a hydrophobic analyte, was studied in this research to see if non-standard microdialysis compounds can be collected. This is important because the pentane resembles hydrophobic signaling molecules in the brain, such as prostaglandins and leukotrienes and can ultimately lead to more information on the regulation of certain hormones in the body. The partition coefficient for pentane in safflower oil/air was calculated to determine if pentane could be successfully used in microdialysis. At the conclusion of this project, pentane was able to be collected and therefore non-standard microdialysis compounds can be collected.

The focus of this research switched from gas phase to solid phase chemistry to further broaden the study. Microdialysis was first completed with a 100 µM stock solution of imipramine hydrochloride. Microdialysis was completed again with the addition of C-18 Silica Magnetic Beads to increase the relative recovery of the analyte. The concentration of the analyte did decrease as expected because the beads adsorbed a portion of the analyte.

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