Date of Graduation
Master of Science in Cell & Molecular Biology (MS)
Second Committee Member
Pure sciences; Biological sciences; Brain; CCL2; Cytokines; IL-6; Immunoassay; Microdialysis
In this thesis, different methods to improve the microdialysis collection procedure for cytokines from brain tissue are presented. The first method was based on stopped flow and results indicating that no significant difference in relative recovery between stopped flow and continuous flow are shown. The second method is an antibody bead-based enhancement method. With the antibody bead-based method, a 3.5 fold increase in the collected concentrations of Chemokine (C-C motif) Ligand 2 (CCL2) were observed. However, there was no significant increase in the in vivo collection efficiency of Interleukin-6 (IL-6) using the antibody enhancement. Finally the development of an in-house created bead assay is discussed for the enhanced collection and quantification for molecules that do not have a commercially available bead-based immunoassay kit. The in-house assay when compared to a commercially available kit for CCL2 showed that a successful assay can be made using the technique employed. Using this method a bead based assay could be made for molecules that do not have these commercially available kits.
Herbaugh, Anthony W., "The Development of an In Vivo Microdialysis Collection Method of Cytokines from Brain Tissue" (2013). Theses and Dissertations. 1018.