Date of Graduation
Master of Science in Chemistry (MS)
Chemistry & Biochemistry
Second Committee Member
Quantum dots are a valuable tool in many research applications and commercial development. Their applications are far reaching and as such have become a topic of great interest. One of their greatest assets is the ability to utilize them as water soluble fluorescent labels. Rendering the nanocrystals water soluble can be accomplished in several ways, but one very popular method is by capping them with water-soluble multi-functional organic ligands. However, these ligands, when attached to the quantum dot surface, are labile and can be exchanged which can be problematic when using the quantum dots in research applications. This projects investigates the mechanisms of the exchange of surface bound ligands by employing modified fluorescent dyes as probes. The kinetics of the exchange is quantified by studying the quenching of the quantum dot signal as a function of time caused by bound dyes through Förster resonance energy transfer. Quantum dots with various ligands of different size, charge, and coordination are exposed to water-soluble dyes of different charges. Overall, it was discovered that a multi-component mechanism of exchange is present. The rate of the first very fast components provide insight into the initial binding of ligands to the surface, while the longer component(s) provides information about the exchange of ligands by dyes on the surface of the quantum dot.
McBride, Randee Jean, "FRET Study of Ligand Binding and Exchange Kinetics on the Surface of CdSe/ZnS Quantum Dots" (2015). Theses and Dissertations. 1267.