Date of Graduation

5-2014

Document Type

Thesis

Degree Name

Master of Science in Chemical Engineering (MSChE)

Degree Level

Graduate

Department

Chemical Engineering

Advisor

Ranil Wickramasinghe

Committee Member

Robert Beitle

Second Committee Member

Wen Zhang

Keywords

Bacteriophages, Protein Separation, Ultrafiltration, Virus Separation

Abstract

Industrial production of protein therapeutics demand rigorous testing and clearance of viruses. The U.S. Food and Drug Administration dictate the purity of pharmaceuticals with regards to viral contamination. As this testing is time consuming and expensive using mammalian cells and viruses, bacteriophages may provide a faster and cheaper alternative for membrane filtration processes. We used ultrafiltration membranes to filter protein solutions with viruses. Two bacteriophages were tested against membranes with two different pore sizes. These membranes were then tested by inverting the membrane's orientation. Flux measurements and log virus removal data were taken. Flux and log virus removal were seen to be slightly higher than published data for mammalian virus analogue minute virus of mice. Future testing would allow for more precise evaluation, but data suggests bacteriophages provide similar results to mammalian data.

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