Date of Graduation
5-2020
Document Type
Thesis
Degree Name
Master of Science in Chemical Engineering (MSChE)
Degree Level
Graduate
Department
Chemical Engineering
Advisor/Mentor
Shannon Servoss
Committee Member
Lauren Greenlee
Second Committee Member
Ranil Wickramasinghe
Third Committee Member
Julie Stenken
Keywords
Antigen, Desorption, GFP, Immunosensor, Peptoid, QCM
Abstract
In this work, we have made and characterized a pair of immunobiosensors for detecting the green fluorescent protein (GFP) in an aqueous matrix. An anti-GFP antibody-based biosensor was assembled to detect GFP, while a novel peptoid (N-substituted oligomers of glycine designated as IOS-1) biosensor was also assembled for GFP detection. A quartz crystal microbalance (QCM) gold sensor was used as the supporting substrate for self-assembly of the immunobiosensors. Gravimetric measurements of the QCM gold sensor during immunobiosensor construction and operation were available in real-time using a QCM instrument. X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and Fluorescence microscopy were used to characterize the immunobiosensors. Dose-dependent calibration curves were developed to contrast the performance of the peptoid immunobiosensor and the antibody-based immunobiosensor. The sensitivity of the biosensors shows that the peptoid could detect GFP at 8 nM, unlike the antibody immunobiosensor, which starts to measurably detect GFP at 40 nM. IOS-1 peptoid immunobiosensor had more adsorption capacity for GFP than the antibody-based immunobiosensor and could be reused through multiple adsorption/ desorption cycles. The peptoid immunobiosensor had a binding constant of 2.197 x 10(7) M(-1) with GFP.
Citation
Isu, S. (2020). Peptoid and Antibody-based GFP Sensors. Graduate Theses and Dissertations Retrieved from https://scholarworks.uark.edu/etd/3918
Included in
Amino Acids, Peptides, and Proteins Commons, Biochemical and Biomolecular Engineering Commons, Membrane Science Commons, Polymer and Organic Materials Commons