Date of Graduation
Bachelor of Science
Committee Member/Second Reader
Committee Member/Third Reader
Methanoperedens nitroreducens (MPEBLZ), an archaeal methanotroph and close relative of Methanosarcina acetivorans, contain numerous cytochrome c proteins. However, difficulty in using these organisms as a model for cytochrome cresearch has created a pressure to express cytochrome c proteins in an organism that is much easier to work with. A punitive cytochrome c protein (MPEBLZ_04274) from M. nitroreducens was attempted to be cloned into a model methanogen M. acetivorans as well as Escherichia coli. Cytochrome c proteins are important for many metabolic processes within anaerobic archaea. In order for a mature cytochrome c to be formed heme must be translocated out of the cell and covalently attached to thiol bonds within CXXCH binding motifs within the protein. Preliminary data suggests that thioredoxin system components, which catalyzes redox reactions within the cell, may be important for the insertion of heme in cytochrome c proteins. To investigate this, CRISPR-dCas9 targeted repression of trx3, trx6, and trx7 was carried out to elucidate the potential impact that these thioredoxins have on the maturation of cytochrome c proteins as well as the ability of the cell to overcome general oxidative stressors.
Methanosarcina acetivorans, cytochrome c, thioredoxin, oxidative stress, CRISPR-cas9
Wojciechowski, B. (2020). Understanding Cytochrome c Maturation in Anaerobic Archaea. Biological Sciences Undergraduate Honors Theses Retrieved from https://scholarworks.uark.edu/biscuht/28