Date of Graduation
Bachelor of Science
Chemistry & Biochemistry
Committee Member/Second Reader
Ceballos, Ruben Michael
Committee Member/Third Reader
Chronic wounds pose a major problem in the United States with an estimate of twenty-five million dollars a year spent on associated treatments. Growth factors can be used as a potential treatment for chronic wounds since they promote cell proliferation and angiogenesis. This study employs one specific growth factor, fibroblast growth factor 2 (FGF2) so that it could potentially be used in future treatment. Wild-type FGF2 is thermally unstable, and it has a mean elimination time of 7.6 hours. This study attempted to improve upon its stability through a mutation on the heparin binding loop. The mutation performed was K134E. This mutation decreased the number of electrostatic interactions present on the heparin-binding loop.
FGF2 was first be attained through the large-scale expression of glutathione-S- transferase-fibroblast growth factor 2 (GST-FGF2). GST-tag was then cleaved from GST-FGF2 by thrombin digestion. The stability and structure were studied through differential scanning calorimetry, circular dichroism, and florescence. This process is a low-cost method since GST-FGF2 was purified from an E. coli culture containing the mutant plasmid
Even though both wild-type and mutant protein were successfully purified at least once, there was not enough protein purified for neither to conduct all characterization studies. A purification procedure was established, and it is possible that the mutant has greater thermal stability than the wild-type but further studies are still needed to make a definitive conclusion.
Chronic wounds, cell proliferation, fibroblast growth, heparin-binding loop
Kaur, M. (2022). The Design of a Efficient Production and Purification of Fibroblast Growth Factor 2. Chemistry & Biochemistry Undergraduate Honors Theses Retrieved from https://scholarworks.uark.edu/chbcuht/32
Available for download on Friday, May 05, 2023