Date of Graduation
5-2023
Document Type
Thesis
Degree Name
Bachelor of Science in Chemistry
Degree Level
Undergraduate
Department
Chemistry & Biochemistry
Advisor/Mentor
Fan, Chenguang
Committee Member/Reader
Girodat, Dylan
Committee Member/Second Reader
Yuchun, Du
Committee Member/Third Reader
D'Eugenio, Daniela
Abstract
Isocitrate dehydrogenase is an important enzyme in the citric acid cycle where it catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate. While there are three isoforms of isocitrate dehydrogenase (IDH1, IDH2, and IDH3), this research will focus on IDH1. The phosphorylation of isocitrate dehydrogenase is a process that has been linked to the formation of both luminal-like and basal-like breast cancer. Despite these correlations, the mechanisms that cause breast cancer development are unknown. To examine this, an enzyme activity assay for each phosphorylation variant and crystallization were conducted. The results of these indicate that phosphorylation at each site (IDH1-T77, IDH1-S188, and IDH1-S237) leads to a decrease in isocitrate dehydrogenase activity when compared to the wild type.
Keywords
IDH1, breast cancer
Citation
Smith, H. (2023). Studying the Phosphorylation of Isocitrate Dehydrogenase in Humans. Chemistry & Biochemistry Undergraduate Honors Theses Retrieved from https://scholarworks.uark.edu/chbcuht/40