Date of Graduation

5-2023

Document Type

Thesis

Degree Name

Bachelor of Science in Chemistry

Department

Chemistry & Biochemistry

Advisor/Mentor

Fan, Chenguang

Committee Member/Reader

Girodat, Dylan

Committee Member/Second Reader

Yuchun, Du

Committee Member/Third Reader

D'Eugenio, Daniela

Abstract

Isocitrate dehydrogenase is an important enzyme in the citric acid cycle where it catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate. While there are three isoforms of isocitrate dehydrogenase (IDH1, IDH2, and IDH3), this research will focus on IDH1. The phosphorylation of isocitrate dehydrogenase is a process that has been linked to the formation of both luminal-like and basal-like breast cancer. Despite these correlations, the mechanisms that cause breast cancer development are unknown. To examine this, an enzyme activity assay for each phosphorylation variant and crystallization were conducted. The results of these indicate that phosphorylation at each site (IDH1-T77, IDH1-S188, and IDH1-S237) leads to a decrease in isocitrate dehydrogenase activity when compared to the wild type.

Keywords

IDH1, breast cancer

COinS