In vitro Microdialysis Sampling Collection of Volatile Organic Compounds (VOC's), Dodecafluoropentane (DDFP) and Isoflurane
Date of Graduation
Master of Science in Chemistry (MS)
Chemistry & Biochemistry
Julie A. Stenken
Second Committee Member
Death by stroke occurs every four minutes to human beings. Strokes cause necrosis within the tissue of the brain due to deprivation of oxygen. Perfluorocarbons have the ability to transport oxygen to tissue and in return decrease cell death. Dodecafluoropentane (DDFP) is a volatile fluorocarbon and collection in vivo can be a challenge since this compound evaporates at room temperature. There is currently not an efficient collection method in vivo for compounds that are volatile. Without a method to collect DDFP it is impossible to be approved for clinical use since exact concentrations of the drug within the body will be unknown. The current in vitro work demonstrates that microdialysis can collect volatile organic compounds, isoflurane (a standard inhalation anesthetic) and DDFP. Different perfusion fluids and flow rates were tested for optimal analyte collection through the microdialysis membrane. Instead of utilizing an aqueous perfusion fluid safflower oil and air was passed through the microdialysis probe. The perfusion fluid and flow rate of choice for isoflurane sampling was safflower oil at 0.5 µL/min, respectively. For DDFP there was no significance in flux between flow rates and air was a more suitable perfusate. Since oil was a potential candidate as a perfusion fluid through the microdialysis probe, the oil/air partition coefficients (KOil/Air) were calculated. KOil/Air was determined because the analyte in the air phase will partition into the oil phase that is being perfused through the microdialysis probe. The average calculated KOil/Air for Isoflurane and DDFP was 10.62 and 0.53, respectively. These values lead to isoflurane partitioning more into oil vs. air and DDFP partitioning more into air vs. oil. The new analytical method described here shows that VOC’s can be collected with microdialysis sampling technique and thus serves as a starting point for in vivo collections has been found.
McKinney, V. S. (2015). In vitro Microdialysis Sampling Collection of Volatile Organic Compounds (VOC's), Dodecafluoropentane (DDFP) and Isoflurane. Graduate Theses and Dissertations Retrieved from https://scholarworks.uark.edu/etd/1246