Date of Graduation


Document Type


Degree Name

Master of Science in Crop, Soil & Environmental Sciences (MS)

Degree Level



Crop, Soil & Environmental Sciences


Kris Brye

Committee Member

David Miller

Second Committee Member

Donald Johnson

Third Committee Member

Mike Richardson


Biochar;Contaminated Soil;Heavy Metal;Industrial Hemp;Phytoremediation;Superfund


Soil contamination by cadmium (Cd), lead (Pb), and zinc (Zn) at the Tar Creek Superfund site in Northeast Oklahoma remains a threat to the environment and local ecosystem. Phytoremediation by industrial hemp (Cannabis sativa L.) and the use of biochar (BC) have been independently shown to be effective methods to remediate heavy-metal-contaminated soils. The objective of this greenhouse study was to evaluate the effects of industrial hemp cultivar (‘Carmagnola’ and ‘Jinma’), BC rate (0, 2, 5, and 10% by volume), soil contamination level (low, medium, and high), and their interactions on above- and belowground (AG and BG, respectively) plant dry matter and AG tissue concentrations and uptakes of Cd, Pb, and Zn after 90 days in contaminated soils from the Tar Creek Superfund site. Aboveground dry matter was largest (P < 0.01) in the low-contaminated soil (10.6 g) and smallest in the high-contaminated soil (6.14 g), but was unaffected (P > 0.05) by cultivar. Belowground dry matter was unaffected (P > 0.05) by soil, cultivar, or BC and averaged 2.33 g per plant. Aboveground tissue Cd concentration for the high-contaminated soil was 1.9 and 21.4 times greater (P < 0.01) than the Cd concentration in the medium- and low-contaminated soils, respectively, and was 1.7 times larger (P < 0.01) in the 5 and 10% BC than in the 0 and 2% BC treatments. ‘Carmagnola’ had the numerically largest AG tissue Pb and Zn concentration among both cultivars and was 3.0 times greater (P < 0.01) than in ‘Jinma’ for Pb with 10% BC and 2.4 times greater than in ‘Jinma’ for Zn with 10% BC Results indicate that ‘Carmagnola’ may be a good choice for phytoremediation of Pb-contaminated soils when grown with a BC amendment ≥ 5% (v/v).