Date of Graduation
12-2013
Document Type
Dissertation
Degree Name
Doctor of Philosophy in Cell & Molecular Biology (PhD)
Degree Level
Graduate
Department
Biological Sciences
Advisor/Mentor
Ines Pinto
Committee Member
Gisela F. Erf
Second Committee Member
Ralph Henry
Third Committee Member
Michael Lehmann
Keywords
Biological sciences, Centromeres, Chromatin, Chromosome segregation, Histones
Abstract
Histone H2A plays an important role in chromosomal segregation among parent and daughter cells during mitosis. While it is established that this histone is important in maintaining chromosome number in cell, further work is carried out to explore the role of other histones like H3 and H4 for similar effects. A systematic study is initiated by screening a library based on mutation of different amino acid residues in these histones. This detailed screening identified specific regions within H3 and H4, which are critically important for centromeric function. These histones residing near the DNA entry/exit region of nucleosome effects the functionality of cell cycle protein, Sgo1, which in turn helps maintaining error free cell cycle by sensing proper tension among sister chromatids during anaphase of mitosis. This work shows that a mutation in H3 and H4 at the DNA entry/exit region moderately effects the nucleosome structure and reduces the centromeric and pericentromeric localization of Sgo1.
Citation
Chaudhuri, P. (2013). The Role of Histone H3 and H4 in Centromere Function and Genome Integrity. Graduate Theses and Dissertations Retrieved from https://scholarworks.uark.edu/etd/979