A method and an apparatus for detecting amines or amino acids is disclosed. The apparatus generally includes a capillary electrophoresis separation tube with a post-capillary reactor positioned at the end of the tube to immediately receive separated samples from the tube. The post-capillary reactor includes a solution of Ru(bpy)3 2+ buffered with a base. The post capillary reactor further includes an electrode assembly for providing current to the solution to convert nonluminescing Ru(bpy)3 2+ to luminescing Ru(bpy)3 3+. The method generally includes separating the desired analyte from the sample, contacting the analyte with the solution to produce luminescence, and then photometrically measuring the amount of analyte present as a function of the luminescence.
Chemistry and Biochemistry
Board of Trustees of the University of Arkansas
Bobbitt, D. R., & Jackson, W. A. (1997). Method and apparatus for detection of underivatized amines and amino acids utilizing end column addition of Ru(bpy).sub.3.sup.2+. Patents Granted. Retrieved from https://scholarworks.uark.edu/pat/209