A method and an apparatus for detecting amines or amino acids is disclosed. The apparatus generally includes a capillary electrophoresis separation tube with a post-capillary reactor positioned at the end of the tube to immediately receive separated samples from the tube. The post-capillary reactor includes a solution of Ru(bpy)3 2+ buffered with a base. The post capillary reactor further includes an electrode assembly for providing current to the solution to convert nonluminescing Ru(bpy)3 2+ to luminescing Ru(bpy)3 3+. The method generally includes separating the desired analyte from the sample, contacting the analyte with the solution to produce luminescence, and then photometrically measuring the amount of analyte present as a function of the luminescence.
Chemistry and Biochemistry
Board of Trustees of the University of Arkansas
Bobbitt, Donald R. and Jackson, Warren A., "Method and apparatus for detection of underivatized amines and amino acids utilizing end column addition of Ru(bpy).sub.3.sup.2+" (1997). Patents Granted. 209.