Disclosed is an ATPase-deficient dominant-negative mutant NS3 protein of hepatitis C virus inhibits activity of the wild-type NS3 protein and inhibits replication of hepatitis C virus (HCV). The solved crystal structure of a multi-enzyme NS3 complex on a DNA substrate is also provided. Also provided is a method of inhibiting HCV replication in cells infected with HCV involving administering to the cells a dominant-negative mutant NS3 protein. Also, it provides peptides and agents that inhibit HCV replication and methods of identifying agents that inhibit HCV replication.
Chemistry and Biochemistry
Board of Trustees of the University of Arkansas (Little Rock, AR)
Raney, Kevin D.; Cameron, Craig E.; Dave, Bhuvanesh; Sakon, Joshua; Lu, Jeff Zhiqiang; Mackintosh, Samuel G.; and Jennings, Thomas A., "Compounds and methods for inhibiting hepatitis C virus replication" (2008). Patents Granted. 94.