Disclosed is an ATPase-deficient dominant-negative mutant NS3 protein of hepatitis C virus inhibits activity of the wild-type NS3 protein and inhibits replication of hepatitis C virus (HCV). The solved crystal structure of a multi-enzyme NS3 complex on a DNA substrate is also provided. Also provided is a method of inhibiting HCV replication in cells infected with HCV involving administering to the cells a dominant-negative mutant NS3 protein. Also, it provides peptides and agents that inhibit HCV replication and methods of identifying agents that inhibit HCV replication.
Chemistry & Biochemistry
Board of Trustees of the University of Arkansas (Little Rock, AR)
Raney, K. D., Cameron, C. E., Dave, B., Sakon, J., Lu, J. Z., Mackintosh, S. G., & Jennings, T. A. (2008). Compounds and methods for inhibiting hepatitis C virus replication. Patents Granted. Retrieved from https://scholarworks.uark.edu/pat/94