Date of Graduation
5-2020
Document Type
Thesis
Degree Name
Master of Science in Cell & Molecular Biology (MS)
Degree Level
Graduate
Department
Biological Sciences
Advisor/Mentor
Jiali Li
Committee Member
Timothy Alan Kral
Second Committee Member
Suresh Thallapuranam
Keywords
avidin, cas9, charge, protein, solid state nanopore, structure, shape
Abstract
The shape and charge of a protein play significant roles in protein dynamics in the biological system of humans and animals. Characterizing and quantifying the shape and charge of a protein at the single-molecule level remains a challenge. Solid-state nanopores made of silicon nitride (SiNx) have emerged as novel platforms for biosensing such as diagnostics for single-molecule detection and DNA sequencing. SSN detection is based on measuring the variations in ionic conductance as charged biomolecules translocate through nanometer-sized channels driven by an external voltage applied across the membrane. In this paper, we observe the translocation of asymmetric cylindrical structure CRISPR-Cas9 protein and symmetric cylindrical structure Avidin protein driven by an electric field through the solid-state nanopore. We also observe how glycerol impacts on the time durations and current blockage amplitudes produced by the translocation of two proteins in nanopore by using different glycerol concentrations.
Citation
Li, H. (2020). Characterization of Avidin and Case9 Single Protein Molecules by a Solid-state Nanopore Device. Graduate Theses and Dissertations Retrieved from https://scholarworks.uark.edu/etd/3659