Date of Graduation
Master of Science in Cell & Molecular Biology (MS)
Second Committee Member
Chromatin Remodeler, Chromosome Segregation, Histone, INO80
Chromatin remodeling complexes are multi-protein complexes that regulate the dynamics of the nucleosomes in the genome. The INO80 chromatin remodeling complex participates in varied biological processes such as: transcription, DNA repair, DNA replication and chromosome integrity. It catalyzes the eviction of the H2A.Z variant histone as well as whole nucleosome eviction. This complex is comprised of 15 subunits and the contribution of each to chromosome segregations remains unknown. To evaluate the contribution of each subunit to chromosome segregation, we tested deletion mutants of the non-essential subunits for DNA content and benomyl sensitivity. Also, we assessed members of the SWR1 and NuA4 complexes which relate to the same substrate. Additionally, specific members of the complex were tested for genetic interaction with SGO1. The deletion of INO80, ARP5, ARP8, IES6 and TAF14 cause increased ploidy and increased benomyl sensitivity. Additionally, overexpression of SGO1 suppresses the benomyl sensitivity and possibly protects the cell from diploidization. Overall, specific subunits of the complex play a role in chromosome segregation and defects caused by mutants of INO80 could be alleviated by SGO1-mediated bi-orientation.
Moreno, Jesus, "Involvement of the INO80 Complex in Chromosome Segregation" (2018). Theses and Dissertations. 2753.