Document Type
Patent
Publication Date
11-14-2017
Abstract
'Provided is a separatome-based recombinant peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome, such as that of E. coli, yeast, Bacillus subtilis or other prokaryotes, insect cells, mammalian cells, etc. This platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both, with emphasis on the former. Moreover, the platform provides a computerized knowledge tool that, given separatome data and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies leading to efficient product purification. The separatome-based protein expression and purification platform is an efficient bioseparation system that intertwines host cell expression systems and chromatography.
Department
Biological Sciences; Chemical Engineering
Patent Number
US9816068
Application Number
US 20150139944 A1
Application Published
3-21-2015
Application Filed
10-23-2014
Assignee
Board of Trustees of the University of Arkansas (Little Rock, AR); University of Pittsburgh-of the Commonwealth System of Higher Learning (Pittsburgh, PA)
Citation
Brune, E. M., Beitle, R., Ataai, M. M., Bartlow, P. R., & Henry, R. (2017). Separatome-based protein expression and purification platform. Patents Granted. Retrieved from https://scholarworks.uark.edu/pat/328
Comments
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT AND JOINT RESEARCH AGREEMENT DISQUALIFICATION UNDER THE CREATE ACT (COOPERATIVE RESEARCH AND TECHNOLOGY ENHANCEMENT ACT OF 2004 (CREATE ACT) (PUB. L. 108-453, 118 STAT. 3596 (2004)) This invention was made with government support under grants Nos. 0534836, 0533949, 1237252, 1142101, and 1048911, awarded by the National Science Foundation. The U.S. government has certain rights in the invention.
Ellen M. Brune, Department of Chemical Engineering, University of Arkansas, Fayetteville, AR
Robert Beitle, Jr., Department of Chemical Engineering, University of Arkansas, Fayetteville, AR
Ralph Henry, Department of Biological Sciences, University of Arkansas, Fayetteville, AR