Date of Graduation
Bachelor of Science
Committee Member/Second Reader
Committee Member/Third Reader
Studying DNA interactions is advantageous to developments in the medical field since DNA and its interactions affect our health greatly. In addition, it is important to understand how the bendability of DNA depends on metal ions, which are essential for various fundamental processes in cells. In this work, we investigate the use of FRET and self-assembled bent DNA molecules to quantify DNA interactions with magnesium ions. We measured that the FRET efficiency increased at higher concentrations of magnesium ions. We also ran simulations to further understand the mechanisms. These observations show that magnesium ions increase the stability and flexibility of DNA greatly. Compared to our previous work with gel electrophoresis, the FRET measurements were faster and simpler.
FRET, Bent DNA, Magnesium Ions, DNA interactions
Bullard, K. (2023). The use of Fluorescence Resonance Energy Transfer and Bent DNA to Study DNA and Ion Interactions. Physics Undergraduate Honors Theses Retrieved from https://scholarworks.uark.edu/physuht/13
Available for download on Wednesday, May 01, 2024